This cell proliferation assay is equivalent to Alamar Blue and leverages a non-toxic, ready-to-use reagent based on an improved resazurin formulation for higher linearity. The stabilized chemistry enables real time monitoring of cell metabolic activity without media removal, washing, or cell disruption. Can be used for creating a 10% v/v solution for usage during cell staining.
 
This formulation uniquely preserves linear signal response across a broader cell density range, making this assay more accurate at quantification of proliferation, cytostasis, and compound cytotoxicity in both short and long term culture models. The non-toxic design allows sequential repeated measurement on the same wells — enabling kinetic proliferation tracking and preserving precious primary material. This format is ideal for high throughput screening, automation and regulatory method transfer.